Life Science Buffers
Balanced salt solutions and powders used for molecular, protein, nucleic acid, and cell biology applications including cell culture, electrophoresis, ELISA, and chromatography processes; available in a variety of formulations and grades.
Buffer solutions serve to keep pH (acidity or alkalinity) nearly constant in a variety of chemical and biological applications. For example, a bicarbonate buffer helps maintain the pH of blood, a buffered saline maintains cellular contents at a consistent pH level, and buffers help maintain a narrow pH range for enzymes to function correctly.
Most biological samples used in research are kept at a pH of about 7.4. Common biological buffers used for tissue culture include Dulbeccos phosphate buffered saline (PBS), Tris base, HEPES, MOPS, PIPES, and other formulations developed for specific cell lines or applications. Most buffers used in cell culture are also DNase-, RNase-, and protease-free.
The choice of buffer for a particular biological reaction or site depends on several factors:
- Temperature
- Desired or target pH
- Buffer toxicity (to the system)
- Buffer interactions with other system components
eMolecules 602-25-5 | 1,4-DICHLOROANTHRAQUINONE | AstaTech | MFCD00192002 | 277.100 | C14H6Cl2O2 | 98.000 | Clc1ccc(Cl)c2C(=O)c3ccccc3C(=O)c12 | 0.25g | 475589680
1,4-DICHLOROANTHRAQUINONE | AstaTech | 602-25-5 | MFCD00192002 | 277.100 | C14H6Cl2O2 | 98.000 | Clc1ccc(Cl)c2C(=O)c3ccccc3C(=O)c12 | 0.25g | 475589680
Research Products International Corp MES Monohydrate 5 KG
Buffer for use in plant tissue culture.
- pKa (20°C): 6.15
- pH range: 5.5 - 6.7
Medchemexpress LLC PIPES (monosodium) | 10010-67-0 | 99.6% | 324.35 | 25 G
PIPES (1,4-Piperazinediethanesulfonic acid) monosodium is a pH buffer that can be mixed with another disodium salt form of PIPES. By changing the ratio and total amount of the PIPES monosodium and disodium buffers, the pH and ionic strength of the medium can be changed.
- pH buffer
- Can be mixed with disodium salt form of PIPES to adjust pH and ionic strength
- For research use only
Boston Bioproducts Inc Bicine Buffer (0.5 M, pH 8.0) - 250ml
Bicine Buffer (0 5 M pH 8 0) - 250ml (For Research Use and Further Manufacturing Only)
Medchemexpress LLC Pipes (monosodium) | 10010-67-0 | 99.6% | 324.35 | 10 G
PIPES monosodium (1,4-Piperazinediethanesulfonic acid monosodium) is a pH buffer. It can be mixed with the disodium salt form of PIPES to adjust the pH and ionic strength of the medium by altering the ratio and total amounts of the buffers. This product is intended for research use only.
- Functions as a pH buffer
- Can be mixed with disodium salt form of PIPES
- Allows adjustment of pH and ionic strength
- Intended for research use only
Medchemexpress LLC HEPPSO | 68399-78-0 | 99.9% | 25 G
HEPPSO is a zwitterionic buffer designed for various research applications. It operates within a working pH range of 7.1-8.5 and exhibits a relatively high ability to bind copper(II). With a pKa of 7.84 at a 2.0 mM buffer concentration, it is suitable for use in laboratory settings.
- Zwitterionic buffer
- Working pH range of 7.1-8.5
- High ability to bind copper(II)
- pKa of 7.84 at 2.0 mM buffer concentration
- For research use only
![Research Products International Corp BES [N,N-bis(2-Hydroxyethyl)-2-aminoethanesulfonic acid], 25 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-B32070-25.02.JPG-250.jpg)
Research Products International Corp BES [N,N-bis(2-Hydroxyethyl)-2-aminoethanesulfonic acid], 25 Grams
For use in preparing electrophoresis buffers. pKa (25° C) 7.1. The working pH Range in aqueous solution is 6.4-7.8
![Research Products International Corp HEPPSO [N-(2-Hydroxyethyl) piperazine-N'-(2-hydroxypropanesulfonic acid)], 25 Grams](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00006095-H75070-25.0.JPG-250.jpg)
Research Products International Corp HEPPSO [N-(2-Hydroxyethyl) piperazine-N'-(2-hydroxypropanesulfonic acid)], 25 Grams
HEPPSO (N-(2-Hydroxyethyl)piperazine-N'-(3-propanesulfonic acid)) is a zwitterionic buffering agent commonly used particularly in biochemical and molecular biology research. It is valued for its ability to maintain a stable pH in a variety of biological assays and experiments.
HEPPSO is often preferred for its buffering capacity over a wide pH range, typically between pH 6.8 to 8.2, making it suitable for various applications such as protein purification, enzyme assays, cell culture, and electrophoresis.
Additionally, HEPPSO is less likely to interfere with biological processes compared to some other buffering agents, making it a versatile choice for maintaining optimal conditions in biological experiments.
Mitegen HEPES/ NaOH, 1M, pH7.0, 250 ml, CAS # 7365-45-9
Use our stock solutions directly or as a component in solutions you create in your own lab. We prepare our stock solutions using high quality raw materials, ASTM Type 1 water, and sterile packaging.�Our stock solutions are intended for Research and Development Use Only.
Dojindo Molecular Technologies Inc BES (100G), Good's buffer, CAS: 10191-18-1, pka=7.15, Active pH 6.6-8, made in Japan
In biological experiments, it is important to maintain the pH of the solutions used. Buffers, which are mixtures of weak acids and their appropriate conjugate bases, are commonly used in these experiments. Most biological reactions occur when the buffer is at its effective pH range of 6 to 8. Furthermore, the acids and bases used in the biological reaction should not produce chelates with metal ions. For these reasons, Dr. Good developed several aminoethane and aminopropane sulfonic acids that are now widely used for biological research and analysis. Goods buffers have the following characteristics: 1) High water-solubility. 2) Low cell membrane permeability. 3) Consistent acid-base dissociation constants. 4) Low metal chelating capability. 5) High chemical stability. 6) Low absorption spectra in UV and visible regions.
Gold Biotechnology Inc HEPES Free Acid 5 kg
HEPES is a buffering agent used in biochemistry and molecular biology that was selected and described by Good et al It is a zwitterionic piperazinic buffer that is useful for a pH range of 6 8 8 2 HEPES lacks the ability to form a significant complex with most metal ions and is recommended for use as a non-coordinating buffer in solutions with metal ions
Medchemexpress LLC Mes (2-morpholinoethanesulphonic acid) | 4432-31-9 | 99.85% | 1 ML
MES (2-Morpholinoethanesulphonic acid) is an amphoteric ion buffer designed for various biochemistry and molecular biology applications. It functions effectively within a pH range of 5.5-7.0 and is widely recognized as a Good's buffer, suitable for experiments including cell culture, enzyme activity determination, electrophoresis, and protein studies.
- Amphoteric ion buffer
- Buffer capacity from pH 5.5-7.0
- Ideal for cell culture, enzyme activity determination, electrophoresis, and protein studies
- Solid, white to off-white appearance
- Soluble in water and DMSO
Research Products International Corp PIPES FREE ACID ULTRAPURE, 1 KILOGRAM
Heavy metals: <10 ppm
pka (25°C): 6.8
The working pH range in aqueous solution is 6.5-7.5
Enzo Life Sciences Ada-Tyr-(Ahx)3-(Leu)3-vinyl sulfone (100µg)
A highly potent, cell-permeable proteasome inhibitor that targets all individual active subunits with comparable affinity. This extended peptide-based inhibitor can be radioiodinated, allowing detection of labeled subunits by autoradiography. Purity: ≥97%. Long Term Storage: -20°C.